Multiple mRNAs encode peripherin, a neuronal intermediate filament protein.

摘要: Three cDNA clones of 1.6 (3u), 1.2 (5g) and 0.6 (5b) kbp, specific for peripherin, a neuronal intermediate filament protein (IFP), have been isolated from murine neuroblastoma cell lambda gt11 library by immunoscreening using peripherin antiserum. Antibodies eluted the fusion proteins produced 3u 5g recognize spots on immunoblots. Where they overlap three cDNAs identical sequences. exhibits closest homology to type III IFP cDNAs. is corresponding region 5g, except insertion 96 bp fragment at position junction exons 4 5 in 5b also deletion 62 8 9 S1 mapping experiments performed with probes covering 3' end two unexpected regions show that distinct mRNAs correspond Moreover, products, minor 61 56 kd products addition major 58 are observed when poly(A)+ RNA vitro translated, being translated 3u-selected RNA. The RNAs originate alternative splicing unique gene, thus generating polymorphism peripherin.