Mouse lymphoma L1210 cells acquire a new cystine transport activity upon adaptation in vitro.

摘要: Mouse lymphoma L1210 cells maintained in vitro at a high cell density for certain time period adapted themselves to the environment and were able grow indefinitely. From these cells, more than 30 clones isolated. They all had much higher activity take up cystine original supporting previous view that deficiency of uptake limits survival growth vitro. The one cloned line was characterized. enhanced mainly mediated by Na+-independent, saturable system potently inhibited glutamate some other anionic amino acids, but less aspartate. Such not observed cells. results suggest that, upon adaptation vitro, acquire new transport necessary