What cell wall components are the best indicators for Miscanthus digestibility and conversion to ethanol following variable pretreatments

作者: J. M. M. Adams , A. L. Winters , E. M. Hodgson , J. A. Gallagher

DOI: 10.1186/S13068-018-1066-3

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摘要: Energy crops including Miscanthus provide a storable, portable energy source which can be used to complement wide range of products and generation systems. is predominantly in Europe as combustion material for electricity but also has the potential biochemical conversion due its high yield low-nutrient requirements. The ratio holocellulose (hemicellulose cellulose combined) acid detergent lignin (H:L) within senesced previously been shown indicate relative suitability accessions thermochemical conversion. In this study, was assessed examine use selection aid 20 highly-characterised were saccharified using an enzyme mix determine optimum sugar release. Nine these spanning high, medium low H:L ratios then autoclaved with dilute acid, alkali or water, enzymically hydrolysed fermented produce ethanol. Samples taken throughout process allowed assessments released sugars. Enzymic degradation biomass showed relationship between glucose release, release vice versa. Xylose no such relationship. This maintained following pretreatments saccharification, where compound analysis that all pretreatments, repeatedly had highest releases glucose, xylose arabinose, produced more Release measured compounds increased pretreatment severity ethanol yields from each correlated respective yield, providing assurance any inhibitory generated tolerated by fermentation yeast. Strong correlations seen cell wall components, showing some treatments others. good predictor study individual components correlate well, especially hot water mild pretreatments. conclusion, does not advantages over concentration predicting yields.

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