Isolation and characterization of a macromolecular aggregate associated with bilirubin

摘要: Abstract 1. A macromolecular aggregate associated with bile pigments has been isolated from human gallbladder and hepatic bile, using isopycnic gradient ultracentrifugation in caesium chloride. Sixty-six percent of the total pigments, 66% cholesterol, 69% phospholipids 65% salts present were this polymolecular aggregate. 2. The percentage contribution each components measured macromolecule was 14.5% 16% phospholipids, salts, 3% 0.5% protein. 3. molecular weights varied source being 11000 to 20000 bile; 35000 75000 samples gallbladders containing gallstones; 65000 normal gallbladders. weight concentration bile. Heavier complexes more concentrated samples. 4. It suggested that represented micellar aggregation pigment