Nucleolar function and size in cancer cells.

摘要: We have studied the relationship between nucleolar function and size cell doubling time in cancer cells. Seven human lines characterized by different proliferation rates were used. Nucleolar functional activity was evaluated measuring RNA polymerase I expression of upstream binding factor (UBF), DNA topoisomerase I, fibrillarin, three proteins involved synthesis processing rRNA. Transcriptional strictly related to (r = -0.97; P < 0.001). The quantitative distribution UBF, fibrillarin on Western blots using specific monoclonal antibodies densitometric analysis autoradiographic signals. It found be directly transcriptional 0.89, 0.008 for UBF; r 0.95, 0.001 I; 0.91, 0.004 fibrillarin) inversely -0.87, 0.011 -0.97, -0.91, 0.005 fibrillarin). areas measured automated image toluidine blue-stained values stained structures per 0.94, 0.001) -0.98, same area blue also closely amount UBF 0.92, 0.003), 0.98, 0.001), situ AgNOR Our results demonstrated that cells rRNA are time. Quantitative within represents a cytohistological parameter rapidity proliferation.