Adenovirus Vectors for Cytokine Gene Expression
作者： CARL D. RICHARDS , TODD BRACIAK , ZHOU XING , FRANK GRAHAM , JACK GAULDIE
DOI: 10.1111/J.1749-6632.1995.TB32333.X
关键词:
摘要: Recombinant Adenovirus type 5 constructs containing IL-6 cDNA can be used to infect cells in vitro and obtain a high level of expression secretion into culture media. Furthermore, Ad5-IL-6 viruses also Balb/c mice or Sprague-Dawley rats that is sustained over period 3-5 days. Intratracheal infection was accompanied by dramatic increases virus-encoded mRNA levels rat lung tissue, raised detected bronchoalveolar lavage fluids serum, IL-6-dependent sequelae such as liver acute phase responses. This occurs tissue-specific manner, depending on routes the virus. Rat lungs showed prominent expansion (10 fold numbers) all classes lymphocytes, including B cells, T helper (CD4+) CTL (CD8+) at day 7 after which resolved significantly 12. Thus associated biological effects viral vector mediated over-expression transient nature. Other tissues infected with Ad5 thus induced express selected genes fashion. We are currently examining potential for Ad recombinant cytokine vectors therapy cancer bone marrow reconstitution transplantation. use may have broad application study function possibly future gene transfer approach.
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