Protection of Penaeus monodon from Infection of White spot syndrome virus by DNA Construct Expressing Long Hairpin-RNA Against ICP11 Gene

摘要: A plasmid construct (pICP11-LH) was designed to constitutively express long-hairpin RNA (lhRNA) against icp11 gene, which is reportedly the most highly expressed gene of White spot syndrome virus (WSSV) and likely have an important role in viral pathogenesis. The used singly combination with other similar constructs vp28 vp19. total 6 treatments, T1 (pICP11-LH; 35 μg), T2 (pVP28-LH; T3 (pVP28-LH pVP19-LH; 17.5 μg each), T4 25 μg:10 μg), T5 (pICP11-LH, pVP28-LH 11.5 μg each) T6 (pGFP-LH; 35 μg) were injected intramuscularly into 20 g Penaeus monodon specimens. shrimp challenged WSSV 24 hpi protection efficacy measured terms survival load 15 days after challenge. Appropriate negative positive controls used. offered highest (75%) followed by (67%) groups (58%), while showed 25% protection. In all target specific as estimated single tube kit kept check (10–100 copies), whereas unimmunized it progressed severe infection (>105 copies). spite over 3 times higher expression ICP11 compared VP28, its knockdown pICP11-LH did not offer any protective advantage pVP28-LH, either or combination. Moreover, none combinations bettered administered alone. To investigate concerns about deleterious effect persistence constitutive on growth, a lab-scale 1 month growth study conducted 4 treatments T2, T3, T6, where no difference rate observed controls.