Improvement of methyl orange dye biotreatment by a novel isolated strain, Aeromonas veronii GRI, by SPB1 biosurfactant addition

摘要: Aeromonas veronii GRI (KF964486), isolated from acclimated textile effluent after selective enrichment on azo dye, was assessed for methyl orange biodegradation potency. Results suggested the potential of this bacterium use in effective treatment azo-dye-contaminated wastewaters under static conditions at neutral and alkaline pH value, characteristic typical effluents. The strain could tolerate higher doses dyes as it able to decolorize up 1000 mg/l. When used microbial surfactant enhance biodecolorization, Bacillus subtilis SPB1-derived lipopeptide accelerated decolorization rate maximized slightly efficiency an optimal concentration about 0.025%. In order process efficiency, a Taguchi design conducted. Phytotoxicity bioassay using sesame radish seeds were carried out assess biotreatment effectiveness. effectively dye when inoculated with initial optical density 0.5 0.25% sucrose, 0.125% yeast extract, 0.01% SPB1 biosurfactant, conducting agitation phase 24 h incubation. Germination potency showed increase toward nonoptimized indicating improvement biotreatment. comparing synthetic surfactants, drastic decrease inhibition observed presence CTAB SDS. nonionic Tween 80 had positive effect biodecolorization. Also, studies ensured that removal by be due endocellular enzymatic activities. To conclude, addition bioemulsifier reduced energy costs reducing period, biosurfactant stimulated bacterial method may provide highly efficient, inexpensive, time-saving procedure