Actin involvement in apoptotic chromatin changes of hemopoietic cells undergoing hyperthermia.
作者： F. Luchetti , S. Burattini , P. Ferri , S. Papa , E. Falcieri
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摘要: During apoptosis, cell chromatin undergoes characteristic morphological changes, which have been long described in a variety of experimental models but are mostly not yet understood. The aim the present study was to investigate mechanisms underlying this phenomenon and possible role cytoskeleton, particular actin. chosen apoptotic model were HL60 hemopoietic cells undergoing hyperthermia starting point observation thin filament bundles decondensed their early nuclei. characterization these structures undertaken by cytochemical, fluorescent immunogold techniques, directed actin identification. Taken together, our results suggest, cells, deep rearrangement. Moreover, cytoskeletal component, never normal nucleus, appears one, where it can be found polymerized form, promptly recognizable both conventional electron microscopy. We suggest that, similarly played nuclear matrix interphase mitotic could directly involved rearrangement occurring apoptosis.
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