Safety, immunogenicity and protection of A(H3N2) live attenuated influenza vaccines containing wild-type nucleoprotein in a ferret model.
作者： Daniil A. Korenkov , Karen L. Laurie , Patrick C. Reading , Louise A. Carolan , Kok Fei Chan
DOI: 10.1016/J.MEEGID.2018.06.019
关键词:
摘要: Abstract Live attenuated influenza vaccines (LAIVs) are promising tools for the induction of broad protection from due to their ability stimulate cross-reactive T cells against pathogens. One major targets cytotoxic T-cell immunity is viral nucleoprotein (NP), which relatively conserved among antigenically distant viruses. Nevertheless, a diversity epitope composition has been found in NP protein different lineages A The H2N2 master donor virus currently used as backbone LAIV and six genomic segments encoding internal proteins, A/Leningrad/134/17/57 (MDV Len/17), was isolated 60 years ago. As such, NP-specific induced upon vaccination with classical LAIVs 6:2 genome containing this older might be suboptimal circulating In study, panel H3N2 candidates wild-type genes derived viruses were generated by reverse genetics (5:3 composition). These displayed cold adaptation temperature sensitivity phenotypes MDV Len/17 vitro. both 5:3 compositions replicated similar extent upper respiratory tract ferrets. immunogenic high neutralizing hemagglutination inhibition serum antibody titers detected 21 days after infection. All vaccinated animals protected infection heterologous Thus, safe, can induce cross-protective immunity.
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