RNA Quantitation by Fluorescence-Based Solution Assay: RiboGreen Reagent Characterization

作者: Laurie J. Jones , Stephen T. Yue , Ching-Ying Cheung , Victoria L. Singer

DOI: 10.1006/ABIO.1998.2914

关键词:

摘要: … With our standard assay conditions, the RiboGreen assay offers accuracy and precision of measurement with a linear dynamic range spanning three orders of magnitude—from 1 ng/ml …

参考文章(9)
Zbigniew Darzynkiewicz, Simultaneous analysis of cellular RNA and DNA content. Methods in Cell Biology. ,vol. 41, pp. 401- 420 ,(1994) , 10.1016/S0091-679X(08)61731-8
Judith Markovits, Bernard P. Roques, Jean-Bernard Le Pecq, Ethidium dimer: a new reagent for the fluorimetric determination of nucleic acids. Analytical Biochemistry. ,vol. 94, pp. 259- 264 ,(1979) , 10.1016/0003-2697(79)90357-9
H.S. Rye, J.M. Dabora, M.A. Quesada, R.A. Mathies, A.N. Glazer, Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity. Analytical Biochemistry. ,vol. 208, pp. 144- 150 ,(1993) , 10.1006/ABIO.1993.1020
Cesar Labarca, Kenneth Paigen, A simple, rapid, and sensitive DNA assay procedure. Analytical Biochemistry. ,vol. 102, pp. 344- 352 ,(1980) , 10.1016/0003-2697(80)90165-7
Victoria L. Singer, Laurie J. Jones, Stephen T. Yue, Richard P. Haugland, Characterization of PicoGreen Reagent and Development of a Fluorescence-Based Solution Assay for Double-Stranded DNA Quantitation Analytical Biochemistry. ,vol. 249, pp. 228- 238 ,(1997) , 10.1006/ABIO.1997.2177
David M. Schmidt, Joel D. Ernst, A Fluorometric Assay for the Quantification of RNA in Solution with Nanogram Sensitivity Analytical Biochemistry. ,vol. 232, pp. 144- 146 ,(1995) , 10.1006/ABIO.1995.0025
Jean-Bernard Le Pecq, Claude Paoletti, A new fluorometric method for RNA and DNA determination Analytical Biochemistry. ,vol. 17, pp. 100- 107 ,(1966) , 10.1016/0003-2697(66)90012-1
W.-Y Li, J.-G. Xu, X.-Q. Guo, Q.-Z. Zhu, Y.-B. Zhao, A Novel Fluorometric Method for DNA and RNA Determination Analytical Letters. ,vol. 30, pp. 527- 536 ,(1997) , 10.1080/00032719708001799