Liquid chromatographic-atmospheric pressure chemical ionization mass spectrometric determination of anandamide and its analogs in rat brain and peripheral tissues
作者： Daisuke Koga , Tomofumi Santa , Takeshi Fukushima , Hiroshi Homma , Kazuhiro Imai
DOI: 10.1016/S0378-4347(96)00391-X
关键词:
摘要: A simple and selective method for the determination of anandamide (arachidonoylethanolamide), an endogenous cannabinoid receptor ligand, its analogs with liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS) was developed. The calibration curve standard linear over range 625 fmol-125 pmol per injection (r = 0.999) a precision 1.0% (C.V.) at 25 pmol. detection limit attained 200 fmol signal-to-noise ratio 2. Anandamide were extracted from rat brain peripheral tissues according to Folch, recovery homogenates 67.0-72.6%. applied their in tissues.
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