The effect of temperature on electrochemically driven denaturation monitored by SERS
作者： Evanthia Papadopoulou , Marta Meneghello , Pietro Marafini , Robert P. Johnson , Tom Brown
DOI: 10.1016/J.BIOELECHEM.2015.06.007
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摘要: Scanning the electrochemical potential negative results in gradual denaturation of dsDNA immobilised at a nanostructure gold electrode, DNA melting is monitored by SERS. We demonstrate effect experimental temperature on electrochemically driven (E-melting) carrying out experiments between 10 and 28 °C using two duplexes (20 21 base pairs). Significant dependence for both potentials, Em, steepness curves was found over range to 18 °C. Above were be independent temperature. The relative insensitivity potentials above advantageous application technique because precise control not necessary measurements that are carried around room Conversely below offers way improve discrimination highly similar sequences.
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