Genetic Ecotoxicology III: the Relationship Between DNA Strand Breaks and Genotype in Mosquito Fish Exposed to Radiation

摘要: DNA polymorphism in mosquito fish (Gambusia affinis), as revealed by RAPD (randomly amplified polymorphic DNA) and allozyme analysis, was compared to relative amounts of strand breakage blood liver tissues. Mosquito were exposed radionuclide contamination situ X-rays the laboratory. The types metrics used number bands per individual frequency certain bands. In a previous study, it noted that some instances elevated radionuclide-contaminated sites reference sites. present found median molecular length (MML) (which is inversely proportional amount breakage) correlated several cases individual. addition, for those occurred at higher from radionuclidecontaminated sites, often lower with than without these data obtained on laboratory paralleled field. Furthermore, analysis showed heterozygotes locus nucleoside phosphorylase more prevalent had fewer breaks did homozygotes. These results provide additional evidence changes population genetic structure genotoxicant (radiation) can be detected level.