Endothelium-derived bradykinin: implications for angiotensin-converting enzyme-inhibitor therapy.

摘要: The effects of angiotensin-converting enzyme (ACE) inhibitors on endothelial autacoid formation were determined in human cultured cells and endothelium-intact bovine coronary arteries under resting conditions after stimulation with bradykinin. Incubation moexiprilat or ramiprilat (0.3 μM) caused a maintained increase intracellular calcium [Ca 2+ ] i , which was prevented by the selective B 2 -receptor antagonist Hoe 140 (0.1 μM). Both ACE also significantly enhanced elicited bradykinin (3 nM). In parallel their effect both induced an cyclic GMP (cGMP)