Changes in the composition of the upper respiratory tract microbial community in granulomatosis with polyangiitis.

摘要: Dysbiosis¸ i.e. changes in microbial composition at a mucosal interface, is implicated the pathogenesis of many chronic inflammatory and autoimmune diseases. To assess upper respiratory tract (URT) community patients with granulomatosis polyangiitis (GPA), we used culture-independent high-throughput methods. In this prospective clinical study, nasal swabs were collected from GPA, rheumatoid arthritis (RA, disease control), healthy controls. Nasal bacterial taxa assessed using V3-V4 region 16S rRNA amplicon sequencing. Staphylococcus aureus, Haemophilus influenza, entero- rhinoviruses detected qPCR. Unbiased metagenomic RNA sequencing (UMERS) was performed subset samples to determine relative abundance bacterial, fungal, viral species. A trend toward reduced microbiome diversity GPA compared The richness significantly decreased RA samples. families shifted, increased Planococcaceae Moraxellaceae, Tissierellaceae, Staphylococcaceae, Propionibacteriaceae RA. Further, Corynebacteriaceae, Aerococcaceae observed Significantly more colonization S. aureus seen control H. influenzae also UMERS presence rhinoviral sequences some Thus, our study uncovered URT RA, suggesting that both immunosuppression background affect microbiome. Complex alterations host-microbiome interactions could influence endonasal inflammation GPA.