The infuence of poliovirus infection on RNA synthesis in mammalian cells

摘要: The incorporation of P32 into RNA in ERK cells suspension was examined by centrifuging the extracted a sucrose density gradient. Treatment with Mitomycin C or ultraviolet light reduced synthesis normal cell (30 S, 20 and 4–8 S) to low level. Infecting such poliovirus did not stimulate detectable earlier than 2 hours after adsorption. By 4 single new radioactive peak appeared gradients, an estimated sedimentation constant about 33–35 same as that from purified virus. No intermediate replicative form viral could be distinguished. Infection caused depression their rate within 112 adsorption, followed stimulation S labeling between 3 hours, while 6 labeling, observable uninfected cells, remained depressed. When temperature incubation raised 40° usual 36°, infected died without producing infective progeny. At took place soon infection, but RNA.