Surface display of a glucose binding protein
作者： Kaiming Ye , Sha Jin , Kelly Bratic , Jerome S Schultz
DOI: 10.1016/J.MOLCATB.2003.12.025
关键词:
摘要: Abstract Glucose binding protein (GBP) from Escherichia coli has been widely used to develop minimally invasive glucose biosensors for diabetics. To a cell-based biosensor, it is essential functionally display GBP on the cell surface. In this study, we designed molecular structure outer membrane of E. . We fused with first nine N-terminal residues Lpp (major lipoprotein) and 46–150 OmpA (an ). With design, have successfully displayed surface Using FITC-conjugated Dextran, demonstrated that glucose’s sites surface-displayed were accessible glucose. Furthermore, showed transport in GBP-deficient NM303 could be restored by displaying NM303. 0.51 h −1 specific growth rate was attained NM303/pESDG grown M9 minimal medium supplemented 2 g/l glucose, whereas no observed same medium. Both grew 1 mM fucose. Because an interface between intracellular extracellular events, technique paves way biosensors.
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