Primer‐independent abortive initiation by wheat‐germ RNA polymerase B (II)
作者： Helmut MOSIG , Anton R. SCHAFFNER , Heidemarie SIEBER , Guido R. HARTMANN
DOI: 10.1111/J.1432-1033.1985.TB08931.X
关键词:
摘要: Highly purified RNA polymerase B (II) from wheat germ catalyses the formation of dinucleoside tetraphosphates ribonucleoside triphosphates in absence an oligonucleotide primer or additional protein factors. The reaction requires bivalent cations such as Mn2+ Mg2+ and proceeds linearly for several hours. It is strongly inhibited by 1 μg/ml α-amanitin 2 heparin. strictly depends on addition a specific linear circular DNA template, plasmid pSmaF fragment containing gene nopaline dehydrogenase. Bacteriophage T7 D111 has almost no template activity. start sites dinucleotide synthesis are limited. With dehydrogenase only pppApA pppApU synthesised substantially whereas pppUpU formed trace amounts. No significant observed with other either singly combination two. various regions differ distinctly
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