Synthesis and immunogenicity of the Mycobacterium tuberculosis arabinomannan–CRM197 conjugate
作者： Yunsong Chang , Xin Meng , Yaxin Li , Jianmei Liang , Tingshen Li
DOI: 10.1039/C8MD00546J
关键词:
摘要: Lipoarabinomannan (LAM) is a major structural surface component of Mycobacterium tuberculosis. This study describes the synthesis well-defined lipoarabinomannan specific dodecasaccharide-protein conjugate and immunological studies. Arabinomannan (AM) dodecasaccharide has been efficiently synthesized covalently conjugated to carrier proteins, including cross reactive mutant (CRM197) diphtheria toxoid bovine serum albumin (BSA) for novel neoglycoconjugates, creating potent T-dependent vaccine. Preliminary mice immunization studies on neoglycoconjugate revealed that it could give rise strong IgG antibody titer in at 4.0 μg dose with an aluminum phosphate adjuvant. AM-CRM197 shows potential as excellent candidate new carbohydrate-based vaccine would be capable eliciting protective immune response against
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sci-hub.se 参考文章(37)
Timothy C Barnett, Jin Yan Lim, Amelia T Soderholm, Tania Rivera-Hernandez, Nicholas P West, Mark J Walker, Host-pathogen interaction during bacterial vaccination. Current Opinion in Immunology. ,vol. 36, pp. 1- 7 ,(2015) , 10.1016/J.COI.2015.04.002
Stefan H E Kaufmann, Tuberculosis vaccines--a new kid on the block. Nature Medicine. ,vol. 17, pp. 159- 160 ,(2011) , 10.1038/NM0211-159
P F Barnes, M Yamamura, D Chatterjee, P J Brennan, E Wang, S Lu, J S Abrams, R L Modlin, Cytokine production induced by Mycobacterium tuberculosis lipoarabinomannan. Relationship to chemical structure. Journal of Immunology. ,vol. 149, pp. 541- 547 ,(1992)
Kamar Sahloul, Todd L. Lowary, Development of an Orthogonal Protection Strategy for the Synthesis of Mycobacterial Arabinomannan Fragments. Journal of Organic Chemistry. ,vol. 80, pp. 11417- 11434 ,(2015) , 10.1021/ACS.JOC.5B02083
Thomas Lindenstrøm, Else Marie Agger, Karen S. Korsholm, Patricia A. Darrah, Claus Aagaard, Robert A. Seder, Ida Rosenkrands, Peter Andersen, Tuberculosis Subunit Vaccination Provides Long-Term Protective Immunity Characterized by Multifunctional CD4 Memory T Cells Journal of Immunology. ,vol. 182, pp. 8047- 8055 ,(2009) , 10.4049/JIMMUNOL.0801592
Gérald Larrouy-Maumus, Martine Gilleron, Henrieta Skovierová, Sophie Zuberogoitia, Patrick J. Brennan, Germain Puzo, Mary Jackson, Jérôme Nigou, A glycomic approach reveals a new mycobacterial polysaccharide Glycobiology. ,vol. 25, pp. 1163- 1171 ,(2015) , 10.1093/GLYCOB/CWV061
Swati Singh, Iti Saraav, Sadhna Sharma, Immunogenic potential of latency associated antigens against Mycobacterium tuberculosis Vaccine. ,vol. 32, pp. 712- 716 ,(2014) , 10.1016/J.VACCINE.2013.11.065
Aharona Glatman-Freedman, The role of antibody-mediated immunity in defense against Mycobacterium tuberculosis: Advances toward a novel vaccine strategy Tuberculosis. ,vol. 86, pp. 191- 197 ,(2006) , 10.1016/J.TUBE.2006.01.008
Rajendrakumar Reddy Gadikota, Christopher S. Callam, Ben J. Appelmelk, Todd L. Lowary, Synthesis of oligosaccharide fragments of mannosylated lipoarabinomannan appropriately functionalized for neoglycoconjugate preparation Journal of Carbohydrate Chemistry. ,vol. 22, pp. 459- 480 ,(2003) , 10.1081/CAR-120025322
Siwarutt Boonyarattanakalin, Xinyu Liu, Mario Michieletti, Bernd Lepenies, Peter H. Seeberger, Chemical synthesis of all phosphatidylinositol mannoside (PIM) glycans from Mycobacterium tuberculosis. Journal of the American Chemical Society. ,vol. 130, pp. 16791- 16799 ,(2008) , 10.1021/JA806283E