Metagenomic characterization of 'Candidatus Defluviicoccus tetraformis strain TFO71', a tetrad-forming organism, predominant in an anaerobic-aerobic membrane bioreactor with deteriorated biological phosphorus removal.

摘要: Summary In an acetate-fed anaerobic–aerobic membrane bioreactor with deteriorated enhanced biological phosphorus removal (EBPR), Defluviicoccus-related tetrad-forming organisms (DTFO) were observed to predominate in the microbial community. Using metagenomics, a partial genome of predominant DTFO, ‘Candidatus Defluviicoccus tetraformis strain TFO71’, was successfully constructed and characterized. Examining confirmed presence genes related synthesis degradation glycogen polyhydroxyalkanoate (PHA), which function as energy carbon storage compounds. TFO71 ‘Candidatus Accumulibacter phosphatis’ (CAP) UW-1 CAP UW-2, representative polyphosphate-accumulating (PAO), have PHA metabolism-related high homology, but has unique for synthesis, gene regulation granule management. We further discovered encoding DTFO polyphosphate (polyP) suggesting that may synthesize polyP under untested conditions. However, not activate these EBPR conditions because retrieved does contain all inorganic phosphate transporters are characteristic PAOs (CAP UW-1, Microlunatus phosphovorus NM-1 Tetrasphaera species). As first step characterizing EBPR-associated metabolism, this study identifies important differences between PAO contribute community competition deterioration.