Michaelis-Menten kinetics predict the rate of SF6 release from permeation tubes used to estimate methane emissions from ruminants

摘要: The sulphur hexafluoride (SF6) technique used to estimate methane emissions from ruminants involves placement of a permeation tube into the reticulo-rumen animals. releases trace amount SF6 across polytetrafluoroethylene membrane. animals emit in exhaled breath enteric along with gas enabling rate emission be calculated ratio SF6. release individual tubes is generally determined over 60–90 day period immediately prior their dosing experimental However, slowly declines time. This decline can result substantial error unless measurements are made within 30 days or correction applied pre-determined release. research presented here shows that declining described by Michaelis–Menten kinetics for up 800 days, parameters predicted initial mass and loss SF6, thus accurately predict future at least 1 year. two important implications this application rates can: (1) increase accuracy estimation ruminants, (2) extend during which estimates estimated year after insertion reticulo-rumen.