Novel Epigenetic CREB-miR-630 Signaling Axis Regulates Radiosensitivity in Colorectal Cancer
作者： Yan Zhang , Jiang Yu , Hao Liu , Wenhui Ma , Li Yan
DOI: 10.1371/JOURNAL.PONE.0133870
关键词:
摘要: Background miR-630 has been reported to be a modulator of several cancers, but the mechanism by which is it influences radioresistance remains unknown. We aimed identify molecular function miR-630 and its regulatory in colorectal cancer (CRC) cell lines. Methodology Overexpression loss-of-function analyses were performed CRC lines measuring their levels growth apoptosis after ionic radiation (IR). Target genes detected via dual-luciferase assay Western blot. Chromatin immunoprecipitation was carried out transcription factor regulating miR-630, demethylation experiment also conducted. Results expression found positively correlated with radiosensitivity (p<0.05). After IR treatment, induced cells; however, opposite observed when downregulated BCL2L2 TP53RK identified as target depend on these two In addition, evidence showed that CREB regulates level can elevate (p<0.05). Conclusion CREB–miR-630–BCL2L2 comprise novel signaling cascade inducing death.
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