Monolithic silica columns with various skeleton sizes and through-pore sizes for capillary liquid chromatography.

摘要: AbstractReduction of through-pore size and skeleton a monolithic silica column was attempted to provide high separationefficiency in short time. Monolithic columns were prepared have various sizes skeletons ( |1–2 mm) andthrough-pores (|2–8 fused-silica capillary (50–200 mm I.D.). The evaluated HPLC afterderivatization C phase. It possible prepare structures capillaries up 200 18 I.D. from amixture tetramethoxysilane methyltrimethoxysilane. As expected, with smaller domainsize showed higher efficiency pressure drop. High external porosity .80%) large through-poresresulted permeability (K58 ?10 –1.3 214 10 m ) that 2–30 times than packed 212 25- particles. produced plate height about 8–12 withan 80% aqueous acetonitrile mobile phase at linear velocity 1 mm/s. Separation impedance, E, found be as lowas 100 under optimum conditions, value an order magnitude lower reported for conventional packedwith 5-mm Although domain generally resulted separation impedance thelower total performance, the performance beyond limit particle-packedcolumns pressure-driven conditions. 2002 Elsevier Science B.V. All rights reserved.Keywords: columns; Stationary phases, LC; Alkylbenzenes