Pico- and femtosecond laser-induced crosslinking of protein microstructures: evaluation of processability and bioactivity
作者： S Turunen , E Käpylä , K Terzaki , J Viitanen , C Fotakis
DOI: 10.1088/1758-5082/3/4/045002
关键词:
摘要: This study reports the pico- and femtosecond laser-induced photocrosslinking of protein microstructures. The capabilities a picosecond Nd:YAG laser to promote multiphoton excited crosslinking proteins were evaluated by fabricating 2D 3D microstructures avidin, bovine serum albumin (BSA) biotinylated (bBSA). absorption-induced was demonstrated here for first time with non-toxic biomolecule flavin mononucleotide (FMN) as photosensitizer. Sub-micrometer micrometer scale structures fabricated from several different compositions photosensitizer varying average power scanning speed in order determine optimal process parameters efficient photocrosslinking. In addition, retention ligand-binding ability crosslinked shown fluorescence imaging immobilized biotin or streptavidin conjugated labels. surface topography resolution patterns compared results obtained Ti:Sapphire laser. Quite similar grain characteristics comparable feature sizes achieved both sources, which demonstrates utility low-cost microlaser direct writing
sci-hub.se 参考文章(45)
Simion Astilean, Alain Duperray, Olivier Stephan, Monica Iosin, Patrice Baldeck, Microstructuration of protein matrices by laser-induced photochemistry Journal of Optoelectronics and Advanced Materials. ,vol. 9, pp. 716- 720 ,(2007)
Peter T. C. So, Chen Y. Dong, Barry R. Masters, Keith M. Berland, Two-Photon Excitation Fluorescence Microscopy Annual Review of Biomedical Engineering. ,vol. 2, pp. 399- 429 ,(2000) , 10.1146/ANNUREV.BIOENG.2.1.399
Roger J Narayan, Anand Doraiswamy, Douglas B Chrisey, Boris N Chichkov, Medical prototyping using two photon polymerization Materials Today. ,vol. 13, pp. 42- 48 ,(2010) , 10.1016/S1369-7021(10)70223-6
B. Kaehr, R. Allen, D. J. Javier, J. Currie, J. B. Shear, Guiding neuronal development with in situ microfabrication Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 16104- 16108 ,(2004) , 10.1073/PNAS.0407204101
Kwang-Sup Lee, Ran Hee Kim, Dong-Yol Yang, Sang Hu Park, Advances in 3D nano/microfabrication using two-photon initiated polymerization Progress in Polymer Science. ,vol. 33, pp. 631- 681 ,(2008) , 10.1016/J.PROGPOLYMSCI.2008.01.001
Bryan Kaehr, Nusret Ertaş, Rex Nielson, Richard Allen, Ryan T. Hill, Matthew Plenert, Jason B. Shear, Direct-write fabrication of functional protein matrixes using a low-cost Q-switched laser. Analytical Chemistry. ,vol. 78, pp. 3198- 3202 ,(2006) , 10.1021/AC052267S
A. Webster, D. Britton, A. Apap-Bologna, G. Kemp, A dye-photosensitized reaction that generates stable protein-protein crosslinks. Analytical Biochemistry. ,vol. 179, pp. 154- 157 ,(1989) , 10.1016/0003-2697(89)90216-9
Frederik Claeyssens, Erol A. Hasan, Arune Gaidukeviciute, Demetra S. Achilleos, Anthi Ranella, Carsten Reinhardt, Aleksandr Ovsianikov, Xiao Shizhou, Costas Fotakis, Maria Vamvakaki, Boris N. Chichkov, Maria Farsari, Three-dimensional biodegradable structures fabricated by two-photon polymerization. Langmuir. ,vol. 25, pp. 3219- 3223 ,(2009) , 10.1021/LA803803M
Irène Wang, Michel Bouriau, Patrice L. Baldeck, Cécile Martineau, Chantal Andraud, Three-dimensional microfabrication by two-photon-initiated polymerization with a low-cost microlaser. Optics Letters. ,vol. 27, pp. 1348- 1350 ,(2002) , 10.1364/OL.27.001348
I. Sakellari, A. Gaidukeviciute, A. Giakoumaki, D. Gray, C. Fotakis, M. Farsari, M. Vamvakaki, C. Reinhardt, A. Ovsianikov, B. N. Chichkov, Two-photon polymerization of titanium-containing sol-gel composites for three-dimensional structure fabrication Applied Physics A. ,vol. 100, pp. 359- 364 ,(2010) , 10.1007/S00339-010-5864-0