Time-dependent activity and expression of glutathione S-transferases in the human colon adenocarcinoma cell line Caco-2.

摘要: The human colon carcinoma cell line Caco-2 was examined for glutathione S-transferase (GST) composition and activity. Freshly seeded cells until 4 days after confluency contain only the placental (Pi) form of transferase. Cells in culture longer periods start to express class-Alpha GST isoenzymes. Confluent 20 or up 90% GST. Class-Mu GSTs are not detectable. activity gradually increases from 564 +/- 28 5381 165 nmol/min per mg protein at day 0 32 respectively. With regard composition, most resemble small-intestinal cells, whereas short-time cultures have characteristics colonic cells. This is very well suited study both vitro properties expression class Alpha Pi GSTs.