Marine Surfaces and the Expression of Specific Byssal Adhesive Protein Variants in Mytilus.

摘要: Mytilus foot protein-3 (Mfp-3) is a highly polymorphic protein family located in the byssal adhesive plaques of blue mussels. Previous evidence suggested that deposition selected Mfp-3 variants might be influenced by type surface to which mussel attaches; therefore, we undertook rigorously investigate whether correlation exists between and variants. Two hypotheses were tested M. galloprovincialis edulis. One hypothesis was individual mussels deposit specific on different surfaces. Laser desorption-ionization mass spectrometry used detect underside plaques. The other transcription Mfp-3s induced This measured using denaturing gradient gel electrophoresis separate closely related amplified complementary DNAs among attached stainless steel, glass, or polyethylene Band stabs several cDNAs sequenced. results clearly showed secreted similar suite onto plastic, steel. Likewise, expression cDNA transcripts revealed no clear messenger RNA surface. Thus, secretion do not appear surface-induced. These underscore importance following rather than populations studies.