Serum activity and hepatic localization of Superoxide dismutase in alcoholics
作者： Takao Inagaki , Satoshi Takiya , Koji Ikuta , Tatsuhisa Sasaki , Katsuhisa Kato
DOI: 10.1007/BF02774325
关键词:
摘要: A series of investigations was conducted to trace serum Superoxide dismutase (SOD) activities by ELISA and localizations hepatic tissue SOD the indirect method using peroxidase conjugated antibody, diagnostic physiological significance in 19 alcoholics studied. The values increased significantly both Cu, Zn-SOD 136±18 ng/ml(normally 33±9 ng/ml) Mn-SOD 859±686 84±30 respectively when polyclonal antibody used (P<0.001). increase higher than that Zn-SOD, fluctuations these showed similar tendencies. Meanwhile, (94±50 identified monoclonal also, normal subjects (37±7 On other hand, localization varied, being 63.2% cytoplasmic diffuse type, 42.0% muclear 42.1% vacuolated membrane 15.8% small granular type respectively. Participation ethanol oxidation, protective roles played cell membrane, lysosome nucleic acid harmful free radicals presumed. In addition, mostly (52.7%) extremely variable. From such results, relative or absolute reductions suggested act on development injuries acceleratingly.
springer.com
sci-hub.se 参考文章(21)
Marklund S, Distribution of CuZn superoxide dismutase and Mn superoxide dismutase in human tissues and extracellular fluids. Acta Physiologica Scandinavica. ,vol. 492, pp. 19- 23 ,(1980)
Kuo-Lan Fong, Paul B. McCay, J. Lee Poyer, Bernard B. Keele, Hara Misra, Evidence that Peroxidation of Lysosomal Membranes Is Initiated by Hydroxyl Free Radicals Produced during Flavin Enzyme Activity Journal of Biological Chemistry. ,vol. 248, pp. 7792- 7797 ,(1973) , 10.1016/S0021-9258(19)43258-4
Uzi REISS, David GERSHON, Rat-liver superoxide dismutase. Purification and age-related modifications. FEBS Journal. ,vol. 63, pp. 617- 623 ,(1976) , 10.1111/J.1432-1033.1976.TB10266.X
EW Kellogg, I Fridovich, Superoxide, hydrogen peroxide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system. Journal of Biological Chemistry. ,vol. 250, pp. 8812- 8817 ,(1975) , 10.1016/S0021-9258(19)40745-X
M Ingelman-Sundberg, I Johansson, Mechanisms of hydroxyl radical formation and ethanol oxidation by ethanol-inducible and other forms of rabbit liver microsomal cytochromes P-450. Journal of Biological Chemistry. ,vol. 259, pp. 6447- 6458 ,(1984) , 10.1016/S0021-9258(20)82163-2
Joe M. McCord, Irwin Fridovich, Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN) Journal of Biological Chemistry. ,vol. 244, pp. 6049- 6055 ,(1969) , 10.1016/S0021-9258(18)63504-5
NOBUHIKO NISHIMURA, YOSHIMASA ITO, TETSUO ADACHI, KAZUYUKI HIRANO, MAMORU SUGIURA, SHUNJI SAWAKI, ENZYME IMMUNOASSAY FOR CUPROZINC-SUPEROXIDE DISMUTASE IN SERUM AND URINE Journal of pharmacobio-dynamics. ,vol. 5, pp. 394- 402 ,(1982) , 10.1248/BPB1978.5.394
Ronald C. Reitz, A possible mechanism for the peroxidation of lipids due to chronic ethanol ingestion. Biochimica et Biophysica Acta. ,vol. 380, pp. 145- 154 ,(1975) , 10.1016/0005-2760(75)90001-6
Etta Livneh, Dov Elad, Joseph Sperling, Light-induced free-radical reactions of nucleic acid constituents. Effect of sequence and base--base interactions on the reactivity of purines and pyrimidines in ribonucleotides. Biochemistry. ,vol. 17, pp. 3128- 3133 ,(1978) , 10.1021/BI00608A029
WALTER J. BRUYNINCKX, HOWARD S. MASON, STEPHEN A. MORSE, Are physiological oxygen concentrations mutagenic Nature. ,vol. 274, pp. 606- 607 ,(1978) , 10.1038/274606A0