Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome
作者： Vímac Nolla-Ardèvol , Marc Strous , Halina E. Tegetmeyer
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摘要: A haloalkaline anaerobic microbial community obtained from soda lake sediments was used to inoculate reactors for the production of methane rich biogas. The microalga Spirulina successfully digested by consortium at alkaline conditions (pH 10, 2.0 M Na(+)). Continuous biogas observed and in methane, up 96%. Alkaline medium acted as a CO2 scrubber which resulted low amounts no traces H2S produced hydraulic retention time (HRT) 15 days 0.25 g L(-1) day(-1) organic loading rate (OLR) were identified optimal operational parameters. Metagenomic metatranscriptomic analysis showed that hydrolysis supplied substrate mainly carried out Bacteroidetes "ML635J-40 aquatic group" while hydrogenotrophic pathway main producer methanogenic dominated Methanocalculus.
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uni-bielefeld.de 参考文章(106)
Terry J. McGenity, Dimitry Y. Sorokin, Methanogens and Methanogenesis in Hypersaline Environments Springer. pp. 283- 309 ,(2018) , 10.1007/978-3-319-53114-4_12-1
Rafael R. de la Haba, C. Sánchez-Porro, M. C. Marquez, Antonio Ventosa, Taxonomy of Halophiles Springer Japan. pp. 255- 308 ,(2011) , 10.1007/978-4-431-53898-1_13
S.G. Pavlostathis, Kinetics and Modeling of Anaerobic Treatment and Biotransformation Processes Reference Module in Earth Systems and Environmental Sciences#R##N#Comprehensive Biotechnology (Second Edition). ,vol. 6, pp. 385- 397 ,(2011) , 10.1016/B978-0-08-088504-9.00385-8
Kursad Turksen, Isolation and characterization Humana Press. ,(2006)
J. B. van Lier, A. Tilche, B. K. Ahring, H. Macarie, R. Moletta, M. Dohanyos, L. W. Hulshoff Pol, P. Lens, W. Verstraete, New perspectives in anaerobic digestion. Water Science and Technology. ,vol. 43, pp. 1- 18 ,(2001) , 10.2166/WST.2001.0001
Ludmila Chistoserdova, Is metagenomics resolving identification of functions in microbial communities Microbial Biotechnology. ,vol. 7, pp. 1- 4 ,(2014) , 10.1111/1751-7915.12077
Ronald S. Oremland, Lorraine Marsh, David J. DesMarais, Methanogenesis in Big Soda Lake, Nevada: an Alkaline, Moderately Hypersaline Desert Lake Applied and Environmental Microbiology. ,vol. 43, pp. 462- 468 ,(1982) , 10.1128/AEM.43.2.462-468.1982
Antonio Ventosa, Unusual micro-organisms from unusual habitats: hypersaline environments Cambridge University Press. pp. 223- 254 ,(2006) , 10.1017/CBO9780511754913.015
Raymond C. Loehr, A. C. Anthonisen, E. G. Srinath, T. B. S. Prakasam, Inhibition of nitrification by ammonia and nitrous acid. Journal of Water Pollution Control Federation. ,vol. 48, pp. 835- 852 ,(1976)
Natalya Yutin, Michael Y. Galperin, A genomic update on clostridial phylogeny: Gram-negative spore formers and other misplaced clostridia. Environmental Microbiology. ,vol. 15, pp. 2631- 2641 ,(2013) , 10.1111/1462-2920.12173