An NAM Domain Gene, GhNAC79, Improves Resistance to Drought Stress in Upland Cotton

摘要: Plant-specific NAC proteins comprise one of the largest transcription factor families in plants and play important roles plant development stress response. Gossypium hirsutum L. is a major source fiber, but its growth productivity are limited by many biotic abiotic stresses. In this study, domain gene GhNAC79 was functionally characterized detail, according to information about cotton genome sequences, it located on scaffold42.1, containing three exons two introns. Promoter analysis indicated that promoter contained both basic stress-related elements, especially expressed cotyledon Arabidopsis. A transactivation assay yeast demonstrated activator, activation at C-terminus. The results qRT-PCR proved preferentially later stages fiber development, showed high sensitivity ethylene meJA treatments. Overexpression resulted an early-flowering phenotype Arabidopsis, also improved drought tolerance Arabidopsis cotton. Furthermore, VIGS-induced silencing led drought-sensitive phenotype. summary, positively regulates stress, responds treatments, making candidate for studies