作者: A. Daszuta , G. Chazal , S. Garcia , S. Oleskevich , L. Descarries
DOI: 10.1016/0306-4522(91)90045-P
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摘要: Abstract Serotonin (5-HT) immunocytochemistry was used at the electron microscopic level to examine 5-HT neurons reinnervating and hyperinnervating hippocampus of adult rat, three four months after a total denervation subsequent graft embryonic raphe cells. The study focused on immunostained nerve cell bodies, dendrites axon terminals (varicosities) in core grafts, large single section sampling from CA3 dentate gyrus sector outgrowth, which were systematically compared endogenous innervation same regions described companion paper. shape, size synaptic investment grafted somata their resembled those situ neurons. Clusters small, clear vesicles sometimes seen along these dendrites. axonal varicosities fairly numerous core. A few directly apposed to, or made asymmetrical contact with perikarya, but vast majority showed no indication junctional specialization (synaptic incidence 19%, as stereologically extrapolated for whole varicosities). Occasional myelinated axons also present grafts. In two outgrowth sectors, graft-borne similar size, content, frequency identity appositional elements, irrespective laminar location. Moreover, none parameters significantly different innervation. Notably, spite excessive number, remained inferior 20%. similarity between respective microenvironments supernumerary, normal counterparts could only be explained by random intratissular distribution both hippocampus. Thus, transplantation growth into an abnormal milieu, fact that they hyperinnervated host tissue, appeared committed express particular set intrinsic relational morphological features corresponding characteristics.