A Zebrafish Live Imaging Model Reveals Differential Responses of Microglia Toward Glioblastoma Cells In Vivo.
作者： Lloyd Hamilton , Katy R. Astell , Gergana Velikova , Dirk Sieger
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摘要: Glioblastoma multiforme is the most common and deadliest form of brain cancer. Glioblastomas are infiltrated by a high number microglia, which promote tumor growth surrounding tissue invasion. However, it unclear how microglia glioma cells physically interact if there differences, depending on cell type. Hence, we have developed novel live imaging assay to study microglia-glioma interactions in vivo zebrafish brain. We transplanted well-established human glioblastoma lines, U87 U251, into transgenic lines with labelled macrophages/microglia. Our confocal results show distinct between U87, as well U251 that differ nature. Importantly these do not appear be antitumoral engulf phagocytose cells. Finally, xenotransplants irf8-/- mutant lacks pharmacological inhibition CSF-1 receptor (CSF-1R) confirm prominent role for promoting growth. This new model will an important tool drug screening development future immunotherapeutics targeting within glioma.
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