Type III Secretion Mediated Translocation of Effector Exoenzymes by Pseudomonas aeruginosa

摘要: Intracellular targeting of the Pseudomonas aeruginosa toxins exoenzyme S (ExoS) and T (ExoT) initially results in disruption actin microfilament structure eukaryotic cells. ExoS ExoT are bifunctional cytotoxins, with N-terminal GTPase-activating protein (GAP) C-terminal ADP-ribosyltransferase activities. We show that can modify multiple GTPases Ras superfamily vivo. In contrast, shows no ADP-ribosylation activity towards any tested further examined targets vivo observed modulates several these small GTP-binding proteins, such as Ras, Rap1, Rap2, Ral, Rac1, RhoA Cdc42. suggest is major modulating GTPase function encoded by P. aeruginosa. Furthermore, we GAP abrogates activation RhoA, Cdc42 Rap1.