Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c

摘要: Objective(s): The present work deals with the preparation of nanobioconjugates based on immobilization cytochrome c (cyt c) functionalized multi-wall carbon nanotubes (f-MWCNTs). The effect nanosupport and procedure biochemical structural characteristics immobilized protein was investigated. Methods: The MWCNTs were to provide alkyl chains different length terminal functional groups their surface. cyt achieved through physical adsorption covalent binding. Cyt-c-based nanoconjugates characterized in terms peroxidase activity stability protein, while UV-visible spectroscopy used investigate protein. Results: The loading f-MWCNTs effectively achieved, yields reaching up 77%. higher case non compared that procedure. Immobilized exhibited thermal than native after 24 h incubation at 40oC, it preserved 100% its initial presence a denaturing agent such as H2O2. No significant changes heme microenvironment observed f-MWCNTs. Conclusions: This study has demonstrated are effective supports for c, providing universally applicable platform development bionanoconjugates potential use wide variety fields nanobiocatalysis, biosensing nanomedicine.