An experimental approach to the analysis of mechanisms of meiotic nondisjunction and anaphase lagging in primary oocytes

摘要: The in vivo genomic mutagenicity of colchicine Chinese hamster primary oocytes was analyzed at a carefully chosen dose that did not completely arrest the formation first meiotic spindle but exhibited remarkable ability to induce nondisjunction, very possibly as result inhibition tubulin polymerization. A single 3 µg per gram body weight administered intraperitoneally onset females with normal estrous cycle. Morphologically abnormal secondary one or two extremely large polar bodies occurred frequently, namely, 47 (11.3%) 416 oocytes. Chromosome analysis total 2,124 revealed overall incidence aneuploids increased significantly (P