Implications of combined Ovariectomy/Multi-Deficiency Diet on rat bone with age-related variation in Bone Parameters and Bone Loss at Multiple Skeletal Sites by DEXA

摘要: Background Osteoporosis is a multi-factorial, chronic, skeletal disease highly prevalent in post-menopausal women and influenced by hormonal dietary factors. Because animal models are imperative for diagnostics, the present study establishes evaluates enhanced osteoporosis obtained through combined ovariectomy deficient diet DEXA (dual-energy X-ray absorptiometry) prolonged time period. Material/methods Sprague-Dawley rats were randomly divided into sham (laparotomized) OVX-diet (ovariectomized fed with diet) groups. Different sites scanned at following points: M0 (baseline), M12 (12 months post-surgery), M14 (14 post-surgery). Parameters analyzed included BMD (bone mineral density), BMC content), bone area, fat (%). Regression analysis was performed to determine interrelationships between BMC, BMD, area from M14. Results significantly lower compared rats. The Z-scores below -5 M12, but still decreased Bone percent regression coefficients vs. of increased time. This explained differential change respect progression. Conclusions Combined caused significant reduction nearly 40% loss after 14 months, indicating development severe osteoporosis. An increasing coefficient progression emphasizes as an important parameter, along prediction fracture risk.