Salt tolerance in indica rice cell cultures depends on a fine tuning of ROS signalling and homeostasis.

摘要: Among cereal crops, salinity tolerance is rare and complex. Multiple genes control numerous pathways, which constitute plant's response to salinity. Cell cultures act as model system are useful investigate the can possibly mimic a stress. In present study two indica rice varieties, KS-282 Super Basmati exhibited contrasting sodium chloride (NaCl) stress were used establish cell cultures. The showed salt at 100 mM NaCl. High level of intracellular hydrogen peroxide (H2O2) nitric oxide (NO) observed in sensitive culture for prolonged period compared tolerant cells an extracellular H2O2 burst along with controlled NO signal was seen. To evaluate role inducing death under stress, percentage (CDP) measured after 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) pre-treatment. CDP reduced significantly both emphasizing NO's possible programmed death. Expression analysis apoplastic NADPH oxidase, i.e. OsRbohA recently characterised OSCA family members OsOSCA 1.2 3.1 done. Intracellular H2O2/NO levels displayed interplay between Ca2+ influx ROS/RNS signal. Detoxifying enzyme (i.e. ascorbate peroxidase catalase) activity considerably higher while superoxide dismutase prominent triggering greater oxidative damage owing presence H2O2. Salt ROS responsive TFs OsSERF1 OsDREB2A expressed exclusively cells. Similarly, expression involved maintaining high [K+]/[Na+] ratio earlier variety. Overall, we suggest that over production, increase important potassium homeostasis play dynamic