Properties of Crystalline α-Glucosidase from Mucor javanicus

摘要: Substrate and inhibitor specificities, transglucosylation action of crystalline α-glucosidase from the mycelia Mucor javanicus have been investigated. The enzyme hydrolyzed maltose, methyl-α-maltoside, soluble starch liberating glucose, but little or not phenyl-α-glucoside, methyl-α-glucoside, sucrose, isomaltose, panose dextran. phenyl-α-maltoside to glucose phenyl-α-glucoside. acted also as a glucosyltransferase when it was incubated with glucosyl donor such maltose. Maltotriose principal product formed catalyzed maltose riboflavin, pyridoxine, esculin rutin. Tris turanose inhibited activity, PCMB EDTA did not. It is suggested that activity closely related histidine residue in active center, inhibition experiments using diazonium-1-H-tetrazole rose bengal.