Evidence for preadipocyte proliferation during culture of subcutaneous and intramuscular adipose tissues from Angus and Wagyu crossbred steers.

摘要: The primary objective of this study was to provide evidence for preadipocyte proliferation during culture adipose tissue explants; a secondary compare the lipogenic activity and cellularity tissues from American Wagyu crossbred steers. Subcutaneous (s.c.) intramuscular (i.m.) were obtained at slaughter 2nd 6th lumbar region loin Angus (n = 10) steers that had been fed 552 d by typical Japanese production standards. Adipose explants incubated 36 h with [3H]thymidine in absence presence aphidicolin (a specific inhibitor genomic DNA replication). Adipocytes liberated collagenase treatment incorporation into measured. Whereas there no significant differences between depots, s.c. i.m. preadipocytes stromal-vascular cells exhibited greater (P < .05) than adipocytes Intramuscular both breeds lower rates lipogenesis acetate before after long-term (36-h) incubation tissue. Furthermore, smaller adipocytes. activities fatty acid synthetase glucose-6-phosphate dehydrogenase less corresponding tissues. There breed types .17) acetate, either or explant culture.(ABSTRACT TRUNCATED AT 250 WORDS)