Doxorubicin-loaded polypeptide nanorods based on electrostatic interactions for cancer therapy
作者： Longlong Zhang , Pei Zhang , Qingyun Zhao , Yongchun Zhang , Longqiao Cao
DOI: 10.1016/J.JCIS.2015.11.008
关键词:
摘要: An amphiphilic anionic polypeptide, methoxypolyethylene glycol-poly (glutamic acid) (mPEG-PGA), was synthesized, characterized and evaluated as a nanocarrier for the cationic anticancer drug doxorubicin hydrochloride (DOX·HCl). The complex self-assembled into nanorods in aqueous solutions via electrostatic interactions exhibited superior loading content (50.8%) efficiency (90.2%). average major axis of drug-loaded approximately 300nm, determined by transmission electron microscopy. vitro release assay showed that pH-sensitivity sustained release. Haemolysis assays demonstrated polypeptide haemocompatible, carrier significantly reduced haemolysis ratio DOX·HCl. pharmacokinetics study DOX-loaded prolonged resident time blood. cytotoxicity cellular uptake resulted higher cell proliferation inhibition level tumour A549 cells than with free circulation enhanced antitumor efficacy shows promise efficient cancer chemotherapy.
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