Use of frozen-thawed cervical tissues in the organ culture system to measure anti-HIV activities of candidate microbicides.
作者： Phalguni Gupta , Deena Ratner , Bruce K. Patterson , Kathy Kulka , Lisa C. Rohan
关键词:
摘要: Cervical tissue-based organ culture system has been used to test the cytotoxicity and antiviral activity of microbicides. One problems using current methods for routine microbicide testing is need continually obtain fresh tissue, which can be limited in access supply. Use frozen stored when available thawed needed, would alleviate constant new tissue. This study was designed explore possibility frozen–thawed cervical tissue microbicides their anti-HIV activity. We provided biochemical, histological, quantitative immunohistochemical data demonstrate integrity system. Significant levels HIV-1 mucosal transmission were noted with both regardless coreceptor usage virus isolate. Furthermore, candidate UC781, β-cyclodextrin, octylglycerol inhibited across mucosa frozen–th...
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