Effect of Puumala hantavirus infection on human umbilical vein endothelial cell hemostatic function: platelet interactions, increased tissue factor expression and fibrinolysis regulator release

摘要: Puumala virus (PUUV) infection causes over 5000 cases of hemorrhagic fever in Europe annually and can influence the hemostatic balance extensively. Infection might lead to hemorrhage, while a recent study showed an increased risk myocardial infarction during or shortly after PUUV infection. The mechanism by which this hantavirus influences coagulation system remains unknown. Therefore we aimed elucidate mechanisms explaining alterations seen primary secondary hemostasis By using low passage isolates infect human umbilical vein endothelial cells (HUVECs) were able show regulation primary- release fibrinolysis regulators. Our main finding was activation due tissue factor (TF) expression leading thrombin generation functional assay. Furthermore, that platelets adhered HUVEC subsequently specifically particles. with did not result von Willebrand they produced more plasminogen activator inhibitor type-1 (PAI-1) compared controls. PAI-1 model formed complexes vitronectin. This is first report reveals potential behind pro-coagulant changes patients, could be TF on provide insight into contribution cell responses regarding pathogenesis.