Studies of the effect of grasshopper abdominal secretion on wound healing with the use of murine model.

摘要: Abstract Ethnopharmacological relevance Grasshopper, belonging to Chorthippus sp., is a widespread insect inhabiting Polish territory. According folk knowledge and tales, the grasshopper abdominal secretion was used by villagers of Central South-West Poland as natural drug accelerating wound healing process. Aim study In reported hypothesis about beneficial properties on hard heal wounds verified. Materials methods The carried out with use murine model (mice C57BL/6). order verify secretion, 8 mm in diameter were formed one side each tested mouse. influence ethanolic extract insects’ process evaluated comparison solution allantoin 30% aqueous ethanol (medium). observation over 14 day period. Finally statistical analysis (ANOVA) highlight differences rate between applied preparations. Moreover, qualitative composition grasshoppers’ studied GC/MS technique. Results During first three days observation, treated healed higher efficiency trend changed from 4th observation. Wounds closuring faster than or ethanol. this part case application, similar. Since 9th experiment measurement size problematic, due crust formation. at 14th study, totally healed. Morphological enabled observe all phases healing. 5th 8th day, infiltration neutrophils mononuclear cells dermis observed, which characteristic for inflammatory phase On experiments, granulation tissue clearly observed groups. Reepithelialization when analytical approach identify 38 compounds hydrophobic hydrophilic character. Among them, 6 amino acids, organic acids their derivatives, sterol, 4 hydrocarbons, 5 carbohydrates, 2 inorganic alcohols, diamine nucleoside identified. Conclusion obtained results recognize secretion. Some identified possess therapeutic described literature. performed vivo proved that application insects accelerates positively verified scientific based ethnopharmacological premises