Transcriptional regulation and mutational analysis of a dctA gene encoding an organic acid transporter protein from Pseudomonas chlororaphis O6.

摘要: A dctA gene encoding a protein with identity to C(4)-dicarboxylic acid/H(+) symporter was cloned from beneficial root colonizer, Pseudomonas chlororaphis O6 (PcO6). Expression of the induced in minimal medium by several organic acids and repressed glucose. Highest expression observed early-logarithmic (log) cells grown on fumarate, acetate or succinate decline as approached late-log growth phase. The transcript accumulated weakly when were malate, but strong benzoate. early-log upon addition glucose detected cell culture aged. dctA-deficient mutant PcO6, constructed marker exchange mutagenesis, did not grow containing succinate, benzoate, fumarate malate delayed. wild-type grew equally citrate, glucose, fructose, sucrose inositol. We conclude that transporter encoded is essential for utilization certain its controlled availability sugars.