Serine protease inhibitors suppress pancreatic endogenous proteases and modulate bacterial neutral proteases

摘要: Pefabloc, Trasylol and Urinary Trypsin Inhibitor (UTI) have been reported to be effective serine protease inhibitors that impair pancreatic endogenous proteases resulting in improved islet yield. Here we evaluated the effect of these on (trypsin, chymotrypsin elastase), bacterial neutral (thermolysin protease) isolation digestion samples. Protease activity was measured using a fluorimetric assay function assessed by dynamic perifusion. Trypsin, elastase were significantly inhibited Pefabloc UTI. showed strong inhibitory effects trypsin but also decreased thermolysin activity. UTI found inhibit increase proteases. Human islets exposed had reduced insulin response, unlike or UTI, which no detrimental secretion. Although an inhibitor proteases, FDA regulatory issues preclude its use clinical application thus process. has greatest potential because it impairs enhances enzymes.