Immunization of mice with vaccinia virus-M2 recombinant induces epitope-specific and cross-reactive Kd-restricted CD8+ cytotoxic T cells.

摘要: Abstract The M2 protein of respiratory syncytial virus (RSV) is a protective antigen in H-2d, but not H-2b or H-2k mice. None the other RSV proteins, excluding surface glycoproteins that induce neutralizing antibodies, mice bearing these haplotypes. Thus, stands alone as nonglycoprotein-protective RSV. The target for murine Kd-restricted cytotoxic T lymphocytes (CTLs), and resistance induced by infection with vaccinia virus-RSV (vac-M2) recombinant mediated CD8+ CTLs. Since nonameric consensus sequence H-2 T-cell epitopes amino acid subgroup A B strains are known, present study sought to identify specific epitope(s) on recognized This was done examining ability four predicted Kd-specific motif peptides an strain sensitize cells lysis pulmonary splenic CTLs obtained from infected vac-M2. following observations were made. First, two sensitized either vac-M2 Second, one peptides, namely 82-90 (M2) peptide, targets at very low peptide concentration (10(-10) 10(-12) M). Third, cold-target competition experiments revealed predominant CTL population this appeared recognize 71-79 cross-reactive manner. Fourth, recognition Kd restricted. Fifth, peptides. findings suggest contains immunodominant epitope consisting residues 82 90 (SYIGSINNI), which shared RSVs.