Nuclear re-localization of Dicer in primary mouse embryonic fibroblast nuclei following DNA damage
作者： Kaspar Burger , Monika Gullerova
DOI: 10.1371/JOURNAL.PGEN.1007151
关键词:
摘要: Dicer is a key component of RNA interference (RNAi) and well-known for its role in biogenesis micro (mi)RNA the cytoplasm. Increasing evidence suggests that mammalian also present active nucleus. We have previously shown phosphorylated human associates with chromatin response to DNA damage processes double-stranded (ds)RNA However, recent study by Much et al. investigated endogenously tagged HA-Dicer both primary mouse embryonic fibroblasts (PMEFs) as well adult homozygous viable fertile mice under physiological conditions concluded murine exclusively cytoplasmic. The authors challenged several findings, reporting functions nuclei. re-investigated this issue applying subcellular fractionation, super-resolution microscopy followed 3D reconstitution, phospho-Dicer-specific antibodies using same PMEF cell line. Our data show small fraction pool, approximately 5%, localises nucleus upon damage. propose localisation dynamic not cytoplasmic, particularly cells exposed
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