Methods for in situ generation of nucleic acid arrays

摘要: Methods of producing nucleic acid arrays using an in situ synthesis protocol are provided, where the includes a plurality cycles, each which includes: (I) monomer attachment step; and (II) functional group generation step, latter may include: (a) oxidation (b) deblocking substeps, optionally capping substep. A feature subject methods is that, following deblock surface, fluid displaced or purged from surface different density, e.g., oxidization wash fluid. Also provided produced methods, as well for use kits that include same.