Effects of dietary selenium and vitamin E on hepatic mixed-function oxidase activities and in vivo covalent binding of aflatoxin B1 in rats.

摘要: Male weanling fischer-344 rats were fed a selenium (Se)-vitamin E (VE) deficient Torula yeast basal diet or that supplemented with graded levels of SE (0.2-6.0 ppm as Na2SeO3) VE (100 iu/kg all-rac-2-tocopheryl acetate), both, for 4 6 weeks. Se deficiency and excess (6.0 ppm) markedly depressed in vivo covalent binding aflatoxin (AFB1) to macromolecules livers killed 2 hours after an i.p. dose 1 mg/kg tritiated AFB1. supplementation had no effect. Prior phenobarbital (PB) treatment generally decreased adducts without changing diet-related trends. Some hepatic enzyme capabilities also measured. Cytochrome b5 content cytochrome c reductase activity unaffected by diet. increased P-450 content, ethylmorphine N-demethylase benz(alpha)pyrene hydroxylase activities; all these levels. (but not deficiency) glucuronyl transferase. PB induction affected groups was more agreement MFO than Adduct formation consistently related transferase activities. The contrasting effects on AFB1 chicks are discussed.