Neurite regeneration from single primary-auditory neurons in vitro.

摘要: Abstract Neurons of the VIII th cranial nerve in lower vertebrates precisely reconnect with their targets after sustaining injury. It is not known, however, whether regenerating neurites are guided entirely by external cues or may also be directed intrinsic mechanisms. To address this issue, single adult primary-auditory neurons were dissected from goldfish and placed an vitro environment, devoid normal complement satellite cells, neighboring neurons, synaptic targets, to observe patterns growth. Because acutely isolated showed little neurite outgrowth, regeneration was enhanced focally crushing 2 24 h prior removal for tissue culture. that regenerated under identical culture conditions growth could categorized into three separate groups based on both morphology patterns. They either 1) remained unbranched (54%), 2) bifurcated trifurcated major branches directly myelinated stump (V-shaped) (19%), 3) a process (Y-shaped), sometimes third, smaller branch (27%). Unbranched V-shaped grew at constant elongation rate, while Y-shaped variably, alternating retractions elongations. Neurite completed uniform time period approximately 15 days despite differences maximum length, latency onset. The branching manner revealed study indicated can reproduce some elements final absence extrinsic cues, capability which ultimately contribute fidelity reconnection.